€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€ *****GENERAL PHARMACOLOGY***** Suh HW Song DK Son KH Wie MB Lee KH Jung KY Do JC Kim YH Antinociceptive mechanisms of dipsacus saponin C administered intracerebroventricularly in the mouse. In: Gen Pharmacol (1996 Oct) 27(7):1167-72 ISSN: 0306-3623 1. Dipsacus saponin C (DSC) administered intracerebroventricularly (i.c.v.) showed an antinociceptive effect in a dose-dependent (from 3.75 to 30 micrograms) manner as measured by the tailflick assay. The antinociception induced by DSC at the dose of 30 micrograms was maintained at least 1 h. 2. Sulfated cholecystokinin (CCK, from 0.1 to 0.5 ng); muscimol (a GABAA receptor agonist, from 50 to 200 ng); MK-801 [(+/-)-5-methyl-10, 11-dihydro-5H-dibenzo (a,d) cyclohepten-5, 10-imine maleate, from 0.1 to 1 microgram], a noncompetitive N-methyl- D-aspartic acid (NMDA) receptor antagonist; or CNQX (6-cyano-7- nitroquinoxaline-2,3-dione, from 0.1 to 0.5 microgram), a non-NMDA receptor antagonist, injected i.c.v. significantly reduced the inhibition of the tail-flick response induced by DSC (30 micrograms) administered i.c.v. However, naloxone (an opioid receptor antagonist, 2 micrograms) or baclofen (a GABAB receptor antagonist, 10 ng) did not affect the inhibition of the tail-flick response induced by DSC. 3. The intrathecal (i.t.) injection of yohimbine (an alpha 2- adrenergic receptor antagonist, from 5 to 20 micrograms) and methysergide (a serotonin receptor antagonist, from 5 to 20 micrograms) but not naloxone (from 2 to 8 micrograms), significantly attenuated inhibition of the tail-flick response induced by DSC (30 micrograms) administered i.c.v. 4. Our results suggest that DSC has an antinociceptive effect when it is administered supraspinally and GABAA, NMDA and non-NMDA receptors, but not opioid and GABAB receptors located at the supraspinal level, may be involved in DSC- induced antinociception. Furthermore, DSC administered supraspinally may produce antinociception by stimulating descending alpha 2- adrenergic and serotonin pathways but not the opioidergic pathway. Registry Numbers: 152406-43-4 (dipsacus saponin C) €€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€ *****JOURNAL OF NATURAL PRODUCTS***** Jung KY Do JC Son KH Triterpene glycosides from the roots of Dipsacus asper. In: J Nat Prod (1993 Nov) 56(11):1912-6 ISSN: 0163-3864 The roots of Dipsacus asper have yielded two new triterpenoid saponins, dipsacus saponins B [1] and C [2], which have been characterized by chemical and spectral means and spectral means as hederagenin-3-O-beta-D-glucopyranosyl-(1-->4)-[alpha-L-rhamnopy ran osyl- (1-->6)]-beta-D-glucopyranosyl-(1-->3)-alpha-L-rhamnopyranosyl- (1-->2)-a lpha- L-arabinopyranoside [1] and hederagenin-3-O-beta-D- xylopyranosyl-(1-->4)- beta-D-glucopyranosyl-(1-->4)-beta-D- glucopyranosyl-(1-->3)-[alpha-L- rhamnopyranosyl-(1-->4)]-alpha-L- rhamnopyranosyl-(1-->2)-alpha-L- arabinopyranoside [2]. Registry Numbers 152406-42-3 (dipsacus saponin B) 152406-43-4 (dipsacus saponin C) €€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€ *****PHYTOCHEMISTRY***** Tomita H Mouri Y An iridoid glucoside from dipsacus asperoides. In: Phytochemistry (1996 May) 42(1):239-40 ISSN: 0031-9422 A new iridoid glucoside, loganic acid-6'-O-beta-D-glucoside, has been isolated from the defatted root of Dipsacus asperoides. Its structure has been elucidated by spectroscopic means as 1S-(1 alpha,4a alpha,6 alpha,7 alpha,7a alpha)-1-[(6-O-beta-D- glucopyranosyl-beta-D- glucopyranosyl)oxyl]1,4a,5,6,7,7a-hexahydro-6-hydr oxy-7- methyl- cyclopenta[c]pyran-4-carboxylic acid. €€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€ *****YAO HSUEH HSUEH PAO [ACTA PHARMACEUTICA SINICA]***** Zhang YW Xue Z [New triterpenoid glycosides from Dipsacus asper Wall] In: Yao Hsueh Hsueh Pao (1991) 26(12):911-7 ISSN: 0513-4870 (Published in Chinese) Two new triterpenoid glycosides were isolated from the ethanol extracts of roots of Dipsacus asper (Wall). Their structures were deduced as 3-O-beta-D-glucopyranosyl (1----3)-alpha-L-rhamnopyranosyl (1----2)-alpha-L-arabinopyranosyl hederagenin 28-O-beta-D- glucopyranosyl (1----6)-beta-D-glucopyranosyl ester (VII) and 3-O- alpha-L-rhamnopyranosyl (1----3)-beta-D-glucopyranosyl (1----3)-alpha- L-rhamnopyranosyl (1----2)-alpha-L-arabinopyranosyl hederagenin 28-O- beta-glucopyranosyl (1----6)-beta-D-glucopyranosyl ester (VIII), based on spectral analysis and chemical evidences as well as results of 2D-HPTLC. €€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€ Zhang YW Xue Z [Studies on the chemical constituents of Dipsacus asper Wall] In: Yao Hsueh Hsueh Pao (1991) 26(9):676-81 ISSN: 0513-4870 (Published in Chinese) Six compounds I-VI were isolated from the roots of Dipsacus asper Wall..Compounds I, II, III and VI were identified as sucrose, daucosterol, beta-sitosterol and akebia saponin D, respectively. On the basis of spectral analysis and chemical evidence, the structures of two new compounds were established as 3-O-(4-O-acetyl)-alpha-L- arabinopyranosyl-hederagenin 28-O-beta-D-glucopyranosyl-(1----6)-beta- D-glucopyranoside (IV) and 3-O-alpha-L-arabinopyranosyl oleanolic acid 28-O-beta-D-glucopyranosyl-(1----6)-beta-D-glucopyranoside (V). €€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€ Zhang YW Xue Z [Structure determination of saponin XI, XII and XIII from Dipsacus asper Wall] In: Yao Hsueh Hsueh Pao (1993) 28(5):358-63 ISSN: 0513-4870 (Published in Chinese) Three new triterpenoid glycosides (XI, XII and XIII) were isolated from the ethanol extract of the roots of Dipsacus asper Wall. Their structures were deduced as 3-O-[beta-D-glucopyranosyl(1-->4)][alpha-L- rhamnopyranosyl (1-->3)]- beta-D-glucopyranosyl(1-->3)-alpha-L- rhamnopyranosyl(1-->2)-alpha- arabinopyranosyl-hederagenin (XI), 28-O- beta-D-glucopyranosyl(1-->6)-beta-D-glucopyranosyl ester of XI (XII) and 3-O-[beta-D-xylopyranosyl(1-->4)-beta-D-glucopyranosyl (1-- >4)][alpha-L-rhamnopyranosyl(1-->3)]-beta-D-glucopyranosyl(1-->3)- alpha-L-rhamnopyranosyl(1-->2)-alpha-arabinopyranosyl-oleanolic.ac id 28-O-beta-D-glucopyranosyl(1-->6)-beta-D-glucopyranosyl ester (XIII), respectively, based on spectral and chemical analysis. €€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€ Zhang YW Xue Z [Structure determination of saponin IX and X from Dipsacus asper Wall] In: Yao Hsueh Hsueh Pao (1992) 27(12):912-7 ISSN: 0513-4870 (Published in Chinese) Two new triterpenoid glycosides (IX and X) were isolated from the ethanol extracts of the roots of Dipsacus asper Wall. Their structures were deduced as 3-O-[beta-D-xylopyranosyl (1-->4)-beta-D- glucopyranosyl(1-->4)] [alpha-L-rhamnopyranosyl (1-->3)]-beta-D- glucopyranosyl(1-->3)-alpha-L-rhamnopyranosyl(1-->2)- alpha- arabinopyranosyl-hederagenin (IX) and 28-O-beta-D-glucopyranosyl(1-- >6)- beta-D-glucopyranosyl ester of IX (X), based on spectral and chemical analysis. Registry Numbers: 508-02-1 (Oleanolic Acid) €€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€