€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€ *****ACTA HISTOCHEMICA***** Ziska P Kindt A Franz H Isolation and characterization of a lectin from garden cress (Lepidium sativuum). In: Acta Histochem (1982) 71(1):29-33 ISSN: 0065-1281 A lectin has been isolated from extracts of garden cress (Lepidium sativum) by affinity chromatography on human immunoglobulin- Sepharose. The lectin reacts with human erythrocytes without specificity for the A, B and 0 blood group. Erythrocytes of animal origin are also agglutinated by the lectin. The hemagglutinating activity is abolished by heating the lectin solution at 70 degrees C or by dialysis against strong acid buffers. The hemagglutination reaction is not inhibited by monosaccharides. Lectin-glycoprotein interactions are described and discussed. €€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€ *****BIOCHIMICA ET BIOPHYSICA ACTA***** Murata T Ishikawa C Chemical, physicochemical and spectrophotometric properties of crystalline chlorophyll-protein complexes from Lepidium virginicum L. In: Biochim Biophys Acta (1981 Apr 13) 635(2):341-7 ISSN: 0006-3002 Two kinds of water-soluble chlorophyll-protein complexes were prepared from leaves of Lepidium virginicum L., one (CP661) from the plant cultivated in a green house from seeds collected near Mono Lake, CA, and the other (CP-663) from a plant collected at Narashino, Chiba, Japan, by ammonium sulfate fractionation followed by column chromatography on DEAE-cellulose and Sephacryl S-200. The chlorophyll . proteins were further purified by crystallization. CP661 has absorption peaks at 661, 468, 439, 419, 380, 339 and 272 nm. CP663 had absorption peaks at 663, 469, 438, 419, 379, 338 and 272 nm. Estimated molecular weights were 78 000 for CP661 and 80 000 for CP663 by gel filtration chromatography and 83 000 for CP661 and 107 000 for CP663 by an equilibrium sedimentation method. 1 mol chlorophyll . protein contained 4 mol chlorophyll a and b with ratios of 1.0 in CP661 and 1.6 to 1.9 in CP663, but no carotenoids. These characters are different from those of chlorophyll-protein complexes which are prepared from the thylakoid membranes of chloroplasts with detergents. Registry Numbers: 1406-65-1 (Chlorophyll) €€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€ Murata T Itoh R Yakushiji E Crystallization of water-soluble chlorophyll-proteins from Lepidium virginicum. In: Biochim Biophys Acta (1980 Nov 5) 593(1):167-70 ISSN: 0006-3002 Water-soluble chlorophyll-proteins were prepared from leaves of Lepidium virginicum, by means of ammonium sulfate fractionation followed by column chromatography on DEAE-cellulose and Sephacryl S- 200. After intensive purification the chlorophyll-proteins were crystallized by dialysis against an ammonium sulfate solution. Registry Numbers: 1406-65-1 (Chlorophyll) €€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€ *****INDIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY***** Vohora SB Khan MS Pharmacological studies on Lepidium sativum, linn. In: Indian J Physiol Pharmacol (1977 Apr-Jun) 21(2):118-20 ISSN: 0019-5499 Pharmacological studies on Lepidium sativum suggested in it the presence of a cardioactive substance, which is unstable in solution, shows tachyphylaxis and probably exerts its actions through adrenergic mechanisms. €€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€ *****JOURNAL OF ANTIBIOTICS***** Anke H Sterner O Steglich W Structure-activity relationships for unsaturated dialdehydes. 3. Mutagenic, antimicrobial, cytotoxic, and phytotoxic activities of merulidial derivatives. In: J Antibiot (Tokyo) (1989 May) 42(5):738-44 ISSN: 0021-8820 The mutagenic activity in the Ames' Salmonella assay, the antimicrobial activities against bacteria, fungi, and algae, the cytotoxic activities against Ehrlich ascitic tumor cells and L1210 cells, and the phytotoxic activities against Lepidium sativum and Setaria italica, of the unsaturated dialdehyde merulidial and six acetylated, hydroxylated, and cyclopropane ring isomerized derivatives of merulidial, are compared. Some possible structure- activity relationships are discussed. Registry Numbers: 68053-32-7 (merulidial) €€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€ Petersen F Zahner H Metzger JW Freund S Hummel RP Germicidin, an autoregulative germination inhibitor of Streptomyces viridochromogenes NRRL B-1551. In: J Antibiot (Tokyo) (1993 Jul) 46(7):1126-38 ISSN: 0021-8820 During germination spores of Streptomyces viridochromogenes NRRL B- 1551 excrete a compound, germicidin, which has an inhibitory effect on the germination of its own arthrospores at a concentration as low as 200 pM (40 pg/ml). At higher concentrations germicidin inhibits porcine Na+/K(+)-activated ATPase and retards the germination of the cress Lepidium sativum. Germicidin is the first known autoregulative inhibitor of spore germination in the genus Streptomyces and was isolated from the supernatant of germinated spores, but also from the supernatant of the submerged culture. Spectroscopic analysis and derivatization reactions revealed germicidin to be 6-(2-butyl)-3- ethyl-4-hydroxy-2-pyrone (C11H16O3). Crude isolates of germicidin from the supernatant of submerged culture, but not from the spores, contained a second, structurally very similar compound (C10H14O3), in which in contrast to germicidin a 2-propyl instead of the 2-butyl chain was bound to C-6 and which did not show any activity in the germination and ATPase assay. The germination assay was evaluated as a new screening model for specifically active compounds. Registry Numbers: 151271-57-7 (germicidin) €€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€ *****JOURNAL OF ETHNOPHARMACOLOGY***** Ramos Ruiz A De la Torre RA Alonso N Villaescusa A Betancourt J Vizoso A Screening of medicinal plants for induction of somatic segregation activity in Aspergillus nidulans. In: J Ethnopharmacol (1996 Jul 5) 52(3):123-7 ISSN: 0378-8741 Knowledge about mutagenic properties of plants commonly used in traditional medicine is limited. A screening for genotoxic activity was carried out in aqueous or alcoholic extracts prepared from 13 medicinal plants widely used as folk medicine in Cuba: Lepidium virginicum L. (Brassicaceae): Plantago major L. and Plantago lanceolata L. (Plantaginaceae); Ortosiphon aristatus Blume, Mentha x piperita L., Melissa officinalis L. and Plectranthus amboinicus (Lour.) Spreng. (Lamiaceae); Cymbopogon citratus (DC.) Stapf (Poaceae); Passiflora incarnata L. (Passifloraceae); Zingiber officinale Roscoe (Zingiberaceae); Piper auritum HBK. (Piperaceae); Schinus terebinthifolius Raddi (Anacardeaceae) and Momordica charantia L. (Cucurbitaceae). A plate incorporation assay with Aspergillus nidulans was employed, allowing detection of somatic segregation as a result of mitotic crossing-over, chromosome malsegregation or clastogenic effects. Aspergillus nidulans D-30, a well-marked strain carrying four recessive mutations for conidial color in heterozygosity, which permitted the direct visual detection of segregants, was used throughout this study. As a result, only in the aqueous extract of one of the plants screened (Momordica charantia) a statistical significant increase in the frequency of segregant sectors per colony was observed, and consequently, a genotoxic effect is postulated. €€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€ Navarro E Alonso J Rodriguez R Trujillo J Boada J Diuretic action of an aqueous extract of Lepidium latifolium L. In: J Ethnopharmacol (1994 Jan) 41(1-2):65-9 ISSN: 0378-8741 An aqueous extract of Lepidium latifolium L. given orally and intraperitoneally considerably enhanced urinary excretion (UV) in rats with respect to control groups. A slight increase in ion excretion was also observed. Other parameters such as specific gravity, nitrite, pH, glucose, ketone bodies, urobilinogen, and blood were also studied. A good correlation for the dosage rat/man for the aqueous extract was achieved. Registry Numbers: 7440-09-7 (Potassium) 7440-23-5 (Sodium) €€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€ *****JOURNAL OF MEDICINAL CHEMISTRY***** Rao KV Beach JW Streptonigrin and related compounds. 5. Synthesis and evaluation of some isoquinoline analogues. In: J Med Chem (1991 Jun) 34(6):1871-9 ISSN: 0022-2623 A series of analogues of streptonigrin, in which the quinoline of ring B is replaced by isoquinoline and the substituted pyridine of ring C is replaced by phenyl, nitrophenyl, aminophenyl, or benzyl functions, have been prepared. Thus, 1-substituted isoquinoline-5,8- diones with 7-amino or 6-(alkylamino) groups were prepared. The various quinones were evaluated for antimicrobial activity against Bacillus subtilis and root-growth inhibitory activity against Lepidium sativum. The effect of specific structural changes on these activities was examined with streptonigrin for comparison. The necessity of an aminoquinone function for activity is confirmed. With regard to the antibacterial activity, the isoquinoline analogues appear to be less active compared to the quinoline derivatives. However, the higher degree of antibacterial activity of the 1- benzylisoquinolines and the 1-nitrophenylisoquinolines compared to the 1-phenyl isoquinolines is noteworthy. In contrast to the results seen with the antibacterial activity, most of the isoquinoline analogues showed activity comparable to, or even higher than, that of streptonigrin in the root-growth inhibition assay. The 1- nitrophenylisoquinolines again appear to be the most active. The equal or greater potency of the benzyl analogue in comparison with the phenyl analogue was unexpected and questions the need for the extended conjugation and the geometry required for metal binding as considered earlier. It also opens up new possibilities for structural variation. Registry Numbers: 119-65-3 (isoquinoline) 3930-19-6 (Streptonigrin) €€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€ *****PLANTA MEDICA***** Hyun JW Shin JE Lim KH Sung MS Park JW Yu JH Kim BK Paik WH Kang SS Park JG Evomonoside: the cytotoxic cardiac glycoside from Lepidium apetalum. In: Planta Med (1995 Jun) 61(3):294-5 ISSN: 0032-0943 [No Abstract Available] Registry Numbers: 143-62-4 (Digitoxigenin) 508-93-0 (evomonoside) €€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€